Identification of polyphenols, glycoalkaloids, and saponins in solanum scabrum berries using HPLC-UV/Vis-MS


Bo Yuan … [et al.]. By: Yuan, Bo


Journal of Food Science 84(2)2019:235-243


Glycoalkaloids. Polyphenols. Saponins. Solanum scabrum. Structural elucidation.


Consumption safety of Solanum scabrum berries is controversial in different cultural practices and evaluation of the toxicity as well as micronutrition value relies on relevant phytochemical study. Thus, this study aimed to systematically profile the phytochemicals in the berries from different genetic sources and maturity. Using a combination of three different and complementary methods of HPLC‐UV/Vis‐MS or MS/MS with acid‐assisted hydrolysis, a total of 54 phytochemicals were identified including polyphenols, saponins and toxic glycoalkaloids. Particularly, a broad range of glycoalkaloids of solasodine and its uncommon or potentially novel hydroxylated and methylated derivatives were reported, with the structure putatively identified based on the known scaffold‐fragmentation pattern. Other identified phytochemicals included phenolic acids of chlorogenic acid and neochlorogenic acid, flavonol glycosides of quercetin and isorhamnetin, anthocyanins of petunidin, malvidin and delphinidin, and saponins of diosgenin and tigogenin